![]() ![]() LncRNA KCNQ1OT1-mediated cervical cancer progression by sponging miR-1270 as a ceRNA of LOXL2 through PI3k/Akt pathway. Circular RNA circHIPK3 is downregulated in diabetic cardiomyopathy and overexpression of circHIPK3 suppresses PTEN to protect cardiomyocytes from high glucose-induced cell apoptosis. Therapeutic exosomal vaccine for enhanced cancer immunotherapy by mediating tumor microenvironment. Long non-coding RNA MIR17HG sponges microRNA-21 to upregulate PTEN and regulate homoharringtonine-based chemoresistance of acute myeloid leukemia cells. Thermo Scientific Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments. N6‑methyladenosine‑induced long non‑coding RNA PVT1 regulates the miR‑27b‑3p/BLM axis to promote prostate cancer progression. 1-Step TMB-Blotting Substrate Solution Thermo Scientific. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.Publishing research using ab65623? Please let us know so that we can cite the reference in this datasheet.Īb65623 has been referenced in 110 publications. The blot was washed 4x with TTBS as before. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Compatible Can replace Amersham ECL Substrate by GE Healthcare and Pierce ECL Substrate by Thermo Fisher without. ![]() The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP.
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